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Test Code LAB1753 Cell Count With Reflex to Differential, CSF

Methodology

 

Hemocytometer/Digital Imaging with DxU 840m Iris and Light Microscopy.

Includes RBC and WBC.  Differential will be performed if WBC is  5 or > /uL

Performing Laboratory

CentraCare Laboratory Services

Specimen Requirements

Specimen must arrive within 1 hour of collection.

 

Specimen Type: Spinal fluid

Container/Tube: Screw-capped, sterile tubes

Specimen Volume: 3 mL divided into 3 tubes each containing 1 mL

Collection Instructions: Label tubes with patient’s name (first and last), medical record number, date and actual time of collection, initials of person collecting specimen, and order in which tubes were collected.

Additional Information:

1. If 4 CSF tubes collected, label tubes by writing the tube number on the label in the following order:

Tube 1: Protein and Glucose

Tube 2. Cell count with differential

Tube 3. Culture and gram stain

Tube 4: Other Testing

2. Some constituents in spinal fluid deteriorate rapidly so analysis must be performed immediately.
3. If malignant cells are encountered, a cytology report will be issued.

Specimen Transport Temperature

Ambient 4 hours/Refrigerated up to 24 hours/Frozen NO

Reference Values

RBC
0 cells/uL
WBC
<1 year: 0-30 cells/uL
1-4 years: 0-20 cells/uL
5-13 years: 0-10 cells/uL
≥14 years: 0-5 cells/uL

 

DIFFERENTIAL (performed if WBC is 5 or>/uL
Mono/Hist
<1 year: 50-90%
>1 year: 16-56%
Lymphocytes
<1 year: 5-35%
>1 year: 28-96%
Neutrophils
<1 year: 0-8%
>1 year: 0-7%

Eosinophils:

<1 year: 0%

> 1 year: 0%

Basophils:

<1 year: 0%

>1 year: 0%

Day(s) Test Set Up

Monday through Sunday

Test Classification and CPT Coding

89050-Cell count/appearance
89051-Cell count differential (if appropriate)