Home
HelpTest Code LAB1407 T-Cell Subsets, Regulatory (Tregs)
Additional Codes
| Mayo Test ID |
| TREGS |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluating patients with clinical features of IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked inheritance) and other primary immunodeficiencies, autoimmune diseases, allergy and asthma, and graft-vs-host disease post-hematopoietic stem cell transplantation
Method Name
Flow Cytometry
Reporting Name
T Cell Subsets, Regulatory (Tregs)Specimen Type
Whole Blood EDTAShipping Instructions
It is recommended that specimens arrive within 24 hours of draw.
Specimens arriving on the weekend may be canceled.
Necessary Information
Ordering physician's name and phone number are required.
Specimen Required
For serial monitoring, we recommend that specimen draws be performed at the same time of day.
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Ambient | 72 hours |
Reject Due To
|
Hemolysis |
Mild OK; Gross reject |
|
Lipemia |
Mild OK; Gross reject |
|
Icterus |
NA |
|
Other |
NA |
Reference Values
The appropriate age-related reference values will be provided on the report.
Interpretation
The lack of regulatory T (Treg) cells is associated with mutations in the FOXP3 gene. Low Tregs are also seen in the context of STAT5b mutations. Reduced Nn Tregs and natural Tregs are likely to predispose to autoimmunity, while reductions in Th3/Tr1 cells may impair oral and peripheral tolerance, also facilitating the development of autoimmunity.
The presence of expanded naive Tregs may indicate a process of malignant transformation, if other clinical features of malignant disease are present.
Increased Tregs in donor stem cell allografts have been associated with a reduced incidence of graft-versus-host disease (ie, mediating a protective effect) after allogeneic stem cell transplantation.
Day(s) and Time(s) Performed
Monday through Friday
Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.
Analytic Time
3 daysSpecimen Retention Time
4 daysTest Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
86359-T cells, total count
86361-Absolute CD4 count
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TREGS | T Cell Subsets, Regulatory (Tregs) | 90413-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 29143 | % Activated CD4+ T cells (4+CD25+) | 13332-2 |
| 29144 | % Natural Tregs | 89320-6 |
| 29145 | % N. Naive Tregs | 89319-8 |
| 29146 | % CD4+CD25-CD127+ (Tr1/Th3) | 89318-0 |
| 29147 | Activated CD4+ T cells (4+CD25+) | 26982-9 |
| 29148 | CD4+CD25+CD127loCD45RO+ (Nat Tregs) | 89316-4 |
| 29149 | CD4+CD25+CD127loCD45RA+ Naive Tregs | 89315-6 |
| 29150 | CD4+CD25-CD127+ (Tr1/Th3) | 89317-2 |
| 29177 | Interpretation | 69052-9 |