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Test Code LAB1315 Mycobacterium tuberculosis Complex, Pyrazinamide Resistance by pncA DNA Sequencing, Varies

Infectious

Additional Codes

Mayo Test ID
MTBPZ

 

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Detection of genotypic resistance to pyrazinamide by Mycobacterium tuberculosis complex isolates

Testing Algorithm

When this test is ordered, the reflex test may be performed and charged.

Method Name

DNA Sequencing

Reporting Name

Mtb PZA Resistance, pncA Sequencing

Specimen Type

Varies


Shipping Instructions


1. See Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and suspected organism identification are required.



Specimen Required


Specimen Type: Organism

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook 7H10 agar slant

Specimen Volume: Isolate

Collection Instructions: Organism must be in pure culture, actively growing.


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Refrigerated 

Reject Due To

Agar plate Reject

Reference Values

Pyrazinamide resistance not detected

Interpretation

Polymorphisms in the pncA gene that have been previously correlated in our laboratory with pyrazinamide (PZA) resistance will be reported as "Mutation was detected in pncA suggesting resistance to pyrazinamide."

 

Wildtype pncA or a silent pncA gene polymorphism (ie, no change in the amino acid translation) will be reported as "No mutation was detected in pncA."

 

New polymorphisms in the pncA gene that have not previously been seen in our laboratory will require additional testing using a reference broth method to determine their correlation with PZA resistance.

Day(s) Performed

Varies

Report Available

7 to 21 days

Specimen Retention Time

1 year

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87153-Mtb PZA Confirmation, pncA Sequence

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTBPZ Mtb PZA Resistance, pncA Sequencing 46245-7

 

Result ID Test Result Name Result LOINC Value
MTBPZ Mtb PZA Resistance, pncA Sequencing 46245-7

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MTBVP Mtb PZA Confirmation, pnc A Sequence No, (Bill Only) No

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.