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Test Code LAB1277 JAK2 V617F Mutation Detection, Bone Marrow

Additional Codes

Mayo Test ID
JAK2M

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Aiding in the distinction between a reactive blood cytosis and a chronic myeloproliferative disorder in bone marrow specimens

Testing Algorithm

See Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation in Special Instructions.

Method Name

Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction (PCR)

Reporting Name

JAK2 V617F Mutation Detection, BM

Specimen Type

Bone Marrow


Shipping Instructions


Specimen must arrive within 7 days (168 hours) of collection.



Specimen Required


Container/Tube:

Preferred: EDTA (lavender top)

Acceptable: ACD (yellow top)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Bone Marrow Ambient (preferred) 7 days
  Refrigerated  7 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

NA

Icterus

NA

Other

Moderately to severely clotted

Reference Values

An interpretive report will be provided.

Interpretation

The results will be reported as 1 of the 2 states:

-Negative for JAK2 V617F mutation

-Positive for JAK2 V617F mutation

 

Positive mutation status is highly suggestive of a myeloid neoplasm, but must be correlated with clinical and other laboratory features for definitive diagnosis.

 

Negative mutation status does not exclude the presence of a myeloproliferative neoplasm or other neoplasm.

 

Results below the laboratory cutoff for positivity are of unclear clinical significance at this time.

Day(s) and Time(s) Performed

Monday through Friday; 12 p.m.

Analytic Time

2 days

Specimen Retention Time

DNA stored for 3 months

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81270-JAK2 (Janus kinase 2) (eg, myeloproliferative disorder) gene analysis, p.Val617Phe (V617F) variant

LOINC Code Information

Test ID Test Order Name Order LOINC Value
JAK2M JAK2 V617F Mutation Detection, BM 72333-8

 

Result ID Test Result Name Result LOINC Value
39723 JAK2 Result 53761-3
31157 JAK2 V617F Mutation Detection, BM 72333-8

Forms

1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.