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General Information:

Web Links:

Mayo Clinic Laboratories

Test Code LAB0293840 Methadone Confirmation, Random, Urine

Additional Codes

 

Mayo Test ID
MTDNU

 

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Monitoring for compliance of methadone treatment for analgesia or drug rehabilitation

 

Assessing compliance with rehabilitation programs by urine measurement of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine

Method Name

Gas Chromatography Mass Spectrometry (GC-MS) Confirmation with Quantitation

Reporting Name

Methadone Confirmation, U

Specimen Type

Urine


Ordering Guidance


1. For situations where chain of custody is required, a Chain-of-Custody Kit (T282) is available. For chain-of-custody testing, order MTDNX / Methadone Confirmation, Chain of Custody, Random, Urine.

2. Additional drug panels and specific requests are available. Call 800-533-1710 or 507-266-5700.

3. If urine creatinine is required or adulteration of the sample is suspected, order ADULT / Adulterants Survey, Random, Urine.



Specimen Required


Supplies: Urine Tubes, 10 mL (T068)

Collection Container/Tube: Plastic urine container

Submission Container/Tube: Plastic, 10-mL urine tube

Specimen Volume: 10 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.


Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reject Due To

Gross hemolysis OK
Gross icterus OK

Reference Values

Negative (Positive results are reported with a quantitative result.)

 

Cutoff concentrations by gas chromatography mass spectrometry:

METHADONE: 100 ng/mL

2-ETHYLIDENE-1,5-DIMETHYL-3,3-DIPHENYLPYRROLIDINE (EDDP): 100 ng/mL

Interpretation

The absolute concentration of methadone and its metabolites found in patient urine specimens can be highly variable and does not correlate with dose. However, the medical literature and our experience show that patients who are known to be compliant with their methadone therapy have ratios of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP):methadone of greater than 0.60.(7)

 

An EDDP:methadone ratio less than 0.090 strongly suggests manipulation of the urine specimen by direct addition of methadone to the specimen.(6)

Day(s) Performed

Tuesday, Thursday

Report Available

3 to 7 days

Specimen Retention Time

14 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

G0480

80358 (if appropriate for select payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTDNU Methadone Confirmation, U 104626-7

 

Result ID Test Result Name Result LOINC Value
56028 EDDP-by GC-MS 104627-5
83129 Methadone-by GC-MS 104628-3
21107 Methadone Interpretation 69050-3
21110 Chain of Custody 77202-0

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.