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Test Code LAB0289058 Epstein-Barr Virus (EBV), Molecular Detection, PCR, Varies

Additional Codes

Mayo Test ID
EBVPV


Necessary Information


Specimen source is required.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis), amniotic, or ocular

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Preferred: Sterile screwcap 5-mL plastic vial

Acceptable: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Container/Tube:

Preferred: Sterile screwcap 5-mL plastic vial

Acceptable: Sterile container

Specimen Volume: 1.5 mL

 

Specimen Type: Swab

Sources: Eye and upper respiratory (nasal, throat)

Supplies:

-Culturette (BBL Culture Swab) (T092)

-M4-RT (T605)

Container/Tube: Multimicrobe media (M4-RT) and Eswabs

Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4 or M5)

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) only

Specimen Volume: 0.5 mL

Additional Information: Clotted specimens will be rejected.

 

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, cornea, etc.

Supplies: M4-RT (T605)

Preferred: Multimicrobe medium (M4-RT)

Acceptable: Sterile container containing 1-2 mL of sterile saline or multimicrobe medium (M4-RT, M4 or M5)

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.


Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens

 

Diagnosis of disease due to EBV

 

This test should not be used to screen asymptomatic patients.

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Reporting Name

Epstein-Barr Virus, PCR, Varies

Specimen Type

Varies

Specimen Minimum Volume

Ocular Fluid, Spinal Fluid: 0.3 mL
Sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis): See Specimen Required
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Calcium alginate-tipped swab
Wood swab
Transport swab containing gel
Formalin-fixed and paraffin-embedded tissues
Heat-inactivated specimens		 Reject

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Detection of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) supports the clinical diagnosis of central nervous system (CNS) disease due to the virus. EBV DNA is not detected in CSF from patients without CNS disease caused by this virus.

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 4 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EBVPV Epstein-Barr Virus, PCR, Varies 5005-4

 

Result ID Test Result Name Result LOINC Value
EBVS Specimen Source 31208-2
618327 Epstein-Barr Virus PCR 5005-4

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.