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Test Code LAB0001882 BCR::ABL1, p190, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Assay, Varies

Additional Codes

Mayo Test ID
BA190

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Therapeutic monitoring of BCR::ABL1 p190 (e1a2)-positive hematologic neoplasms (eg, acute lymphoblastic leukemia, chronic myeloid leukemia)

 

This test should not be used to screen for BCR::ABL1 fusion forms at the time of diagnosis or quantitative therapeutic monitoring of p210 or rare fusion forms.

Testing Algorithm

For information see BCR::ABL1 Ordering Guide for Blood and Bone Marrow.

Method Name

Quantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR)

Reporting Name

BCR/ABL1, p190, Quant, Monitor

Specimen Type

Varies


Ordering Guidance


This test is intended for therapeutic monitoring of patients with known e1a2 BCR::ABL1 (p190) fusion form and should not be used to screen for BCR::ABL1 fusion forms at the time of diagnosis.

 

To screen for BCR::ABL1 fusion forms at diagnosis, order one of the following:

BADX / BCR::ABL1, Qualitative, Diagnostic Assay, Varies

BCRFX / BCR::ABL1 Qualitative Diagnostic Assay with Reflex to BCR::ABL1 p190 Quantitative Assay or BCR::ABL1 p210 Quantitative Assay, Varies.

 

To monitor patients carrying BCR::ABL1 fusion forms coding for p210 (e13/e14a2), order BCRAB / BCR::ABL1, p210, mRNA Detection, Reverse Transcription-PCR (RT-PCR), Quantitative, Monitoring Chronic Myeloid Leukemia (CML), Varies.

 

To monitor patients carrying rare BCR::ABL1 fusion forms coding for e19a2, e13/e14a3, e1a3, e6a2, e19a3, e8a2, e12a2, e6a3, e8a3, and e12a3, order BARQ / BCR::ABL1, Rare Fusion Monitoring, Quantitative, Varies.



Shipping Instructions


Refrigerate specimens must arrive within 5 days of collection, and ambient specimens must arrive within 72 hours of collection. Collect and package specimen as close to shipping time as possible.



Necessary Information


Pertinent clinical history including patient diagnosis of chronic myeloid leukemia or other BCR::ABL1-positive neoplasm information is required.



Specimen Required


Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Whole blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Send whole blood specimen in original tube. Do not aliquot.

3. Label specimen as whole blood.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send bone marrow specimen in original tube. Do not aliquot.

3. Label specimen as bone marrow.


Specimen Minimum Volume

Whole blood: 8 mL; Bone marrow: 2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 5 days PURPLE OR PINK TOP/EDTA
  Ambient  72 hours PURPLE OR PINK TOP/EDTA

Reject Due To

Gross hemolysis Reject
Moderately to severely clotted Reject

Reference Values

The presence or absence of the BCR::ABL1 messenger RNA fusion form producing the p190 fusion protein is reported. If positive, the level is reported as the ratio of BCR::ABL1 (p190) transcript to ABL1 transcript in the form of a percentage.

Interpretation

An interpretive report will be provided which includes the ratio of BCR::ABL1 (p190) transcript to ABL1 transcript in the form of a percentage, if positive.

Day(s) Performed

Monday through Friday

Report Available

4 to 8 days

Specimen Retention Time

Whole blood/Bone marrow: 2 weeks; Extracted RNA 3 months

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81207

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BA190 BCR/ABL1, p190, Quant, Monitor 21823-0

 

Result ID Test Result Name Result LOINC Value
39470 BCR/ABL1 p190 Result No LOINC Needed
MP002 Specimen Type 31208-2
19765 Interpretation 69047-9

Forms

1. Hematopathology Patient Information (T676)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.