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HelpTest Code TLYMF T-Cell Lymphoma, FISH, Tissue
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Detecting a neoplastic clone associated with the common chromosome abnormalities seen in patients with various T-cell lymphomas
Tracking known chromosome abnormalities and response to therapy in patients with T-cell lymphomas
Testing Algorithm
This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.
This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results.
Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.
Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the Clinical Information.
If the patient is being tracked for known abnormalities, indicate which probes should be used.
Panel includes testing for the following abnormalities using the probes listed:
14q32.1 rearrangement, TCL1A
-7/7q-/i(7q), D7S486/D7Z1
+8, D8Z2/MYC
2p23.2 rearrangement, ALK
This assay detects chromosome abnormalities observed in paraffin-embedded tissue samples of patients with T-cell lymphoma. For testing the blood and bone marrow from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.
Method Name
Fluorescence In Situ Hybridization (FISH)
Reporting Name
T-cell Lymphoma, FISH, TsSpecimen Type
TissueAdvisory Information
This test is not appropriate for testing blood or bone marrow samples from patients with T-cell lymphoma, see TLPF / T-Cell Lymphoma, FISH, Blood or Bone Marrow.
Shipping Instructions
Advise Express Mail or equivalent if not on courier service.
Necessary Information
Provide a reason for referral and pathology report with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Lymph node
Preferred: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used
Acceptable: Slides
Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Specimen Type: Solid tumor
Preferred: Tissue block
Collection Instructions: Submit a formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used.
Acceptable: Slides
Collection Instructions: For each probe set ordered, 2 consecutive, unstained, 5 micron-thick sections placed on positively charged slides, and 1 hematoxylin and eosin-stained slide.
Specimen Minimum Volume
For each probe set ordered, 2 consecutive, unstained, 5
micron-thick sections placed on positively charged slides.
Include 1 hematoxylin and eosin-stained slide.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Tissue | Ambient (preferred) | |
| Refrigerated | ||
Reject Due To
No specimen should be rejected.
Reference Values
An interpretive report will be provided.
Interpretation
A neoplastic clone is detected when the percent of cells with an abnormality exceeds the normal reference range for any given probe.
Detection of an abnormal clone is supportive of a diagnosis of a T-cell lymphoma. The specific abnormality detected may help subtype the neoplasm.
The absence of an abnormal clone does not rule out the presence of a neoplastic disorder.
Day(s) and Time(s) Performed
Samples processed Monday through Sunday. Results reported Monday through Friday 8 a.m.-5 p.m.
Analytic Time
7 daysSpecimen Retention Time
Slides and H and E used for analysis are retained by the lab indefinitely. Client provided paraffin blocks and extra unstained slides (if provided) will be returned after testing is complete.Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
88271x2, 88291 – DNA probe, each (first probe set), Interpretation and report
88271x2 – DNA probe, each; each additional probe set (if appropriate)
88271x1 – DNA probe, each; coverage for sets containing 3 probes (if appropriate)
88271x2 – DNA probe, each; coverage for sets containing 4 probes (if appropriate)
88271x3 – DNA probe, each; coverage for sets containing 5 probes (if appropriate)
88274 w/modifier 52 – Interphase in situ hybridization, <25 cells, each probe set (if appropriate)
88274 – Interphase in situ hybridization, 25 to 99 cells, each probe set (if appropriate)
88275 – Interphase in situ hybridization, 100 to 300 cells, each probe set (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TLYMF | T-cell Lymphoma, FISH, Ts | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 52139 | Result Summary | 50397-9 |
| 52141 | Interpretation | 69965-2 |
| 52140 | Result Table | No LOINC Needed |
| 54583 | Result | In Process |
| CG743 | Reason for Referral | 42349-1 |
| 52142 | Specimen | 31208-2 |
| 52143 | Source | 31208-2 |
| 52144 | Tissue ID | 81178-6 |
| 52145 | Method | 49549-9 |
| 55025 | Additional Information | 48767-8 |
| 53832 | Disclaimer | 62364-5 |
| 52146 | Released By | 18771-6 |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| _PBCT | Probe, +2 | No, No (Bill Only) | No |
| _PADD | Probe, +1 | No, No (Bill Only) | No |
| _PB02 | Probe, +2 | No, No (Bill Only) | No |
| _PB03 | Probe, +3 | No, No (Bill Only) | No |
| _IL25 | Interphases, <25 | No, No (Bill Only) | No |
| _I099 | Interphases, 25-99 | No, No (Bill Only) | No |
| _I300 | Interphases, >=100 | No, No (Bill Only) | No |
Forms
If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.