Sign in →

Test Code KIT11 KIT Exon 11, Mutation Analysis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Diagnosis and management of patients with gastrointestinal stromal tumors or melanomas

                      

Identification of a mutation in exon 11 of the KIT gene

Testing Algorithm

This test is performed in conjunction with SLIRV / Slide Review. Additional testing may be performed after review by pathologist. Upon approval from the requesting clinician, PATHC / Pathology Consultation may be added, if determined to be appropriate.

Special Instructions

Method Name

Polymerase Chain Reaction (PCR) and Sequencing

Reporting Name

KIT exon 11, Mutation Analysis

Specimen Type

Varies


Advisory Information


Special stains performed outside Mayo Clinic Laboratories and included with the case may be repeated and charged at the reviewing pathologist's discretion. Testing requested by referring physician may not be performed if deemed unnecessary by Mayo Clinic pathologist.



Necessary Information


A pathology/diagnostic report including a brief history is required. If available, include KIT Immunostain results.



Specimen Required


A quality specimen is essential for evaluation. Submit only tissue containing tumor cells; minimal tissue is required for evaluation.

 

Supplies: Surgical Pathology Packaging Kit (T554) requested, but not required

 

Preferred:

Specimen Type: Formalin-fixed, paraffin-embedded (FFPE) tissue block with a minimum of 60% tumor cell population.

Collection Instructions: Process all specimens into FFPE tissue blocks prior to submission.

 

Acceptable:

Specimen Type: Unstained slides with a minimum of 60% tumor population, slides may be stained and/or scraped.

Slides: A minimum of ten, 4- to 5-micron thick, unstained slides are required.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Frozen 
  Refrigerated 

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Specimens that have been decalcified (all methods); specimens that have not been formalin-fixed, paraffin-embedded; bone marrow in EDTA

Reference Values

An interpretative report will be provided.

Interpretation

Results are reported as positive, negative, or failed. A negative result does not rule out the presence of a mutation.

Day(s) and Time(s) Performed

Monday through Friday; Varies

Analytic Time

14 days

Specimen Retention Time

Unused portions of blocks will be returned. Unused slides are stored indefinitely.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

81272

88381-Microdissection, manual

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KIT11 KIT exon 11, Mutation Analysis 77171-7

 

Result ID Test Result Name Result LOINC Value
54808 Result Summary 50397-9
54809 Result 82939-0
54810 Interpretation 69047-9
54811 Additional Information 48767-8
54812 Reason for Referral 42349-1
54813 Specimen 31208-2
54814 Source 31208-2
54815 Tissue ID 81178-6
54816 Released By 18771-6

Additional Tests

Test ID Reporting Name Available Separately Always Performed
SLIRV Slide Review in MG No, (Bill Only) Yes

Forms

1. Pathology/Cytology Information (T707) in Special Instructions

2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Pathology Test Request Form (T246) (http://www.mayomedicallaboratories.com/it-mmfiles/pathology-request-form.pdf)

Oncology Test Request Form (T729) (http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)