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Test Code DSG13 Desmoglein 1 (DSG1) and Desmoglein 3 (DSG3), IgG Antibodies, Serum

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Preferred screening test for patients suspected to have an autoimmune blistering disorder of the skin or mucous membranes (pemphigus)

 

As an aid in the diagnosis of pemphigus

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

Desmoglein 1 and 3, S

Specimen Type

Serum


Specimen Required


Container/Tube: 

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 1 mL


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  14 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild reject; Gross OK

Icterus

Mild OK; Gross OK

Other

NA

Reference Values

DESMOGLEIN 1

<18 U (negative)

 18-36 U (indeterminate)

≥36 U (positive)

                      

DESMOGLEIN 3

<19 U (negative)

19-37 U (indeterminate)

≥37 U (positive)

Interpretation

Antibodies to desmoglein 1 (DSG1) and desmoglein 3 (DSG3) have been shown to be present in patients with pemphigus. Many patients with pemphigus foliaceus, a superficial form of pemphigus have antibodies to DSG1. Patients with pemphigus vulgaris, a deeper form of pemphigus, have antibodies to DSG3 and sometimes DSG1 as well.

 

Antibody titer correlates in a semiquantitative manner with disease activity in many patients. Patients with severe disease can usually be expected to have high titers of antibodies to DSG. Titers are expected to decrease with clinical improvement.

 

Our experience demonstrates a very good correlation between DSG1 and DSG3 results and the presence of pemphigus. Adequate sensitivities and specificity for disease are documented. However, in those patients strongly suspected to have pemphigus either by clinical findings or by routine biopsy, and in whom the DSG assay is negative, the indirect immunofluorescence (CIFS / Cutaneous Immunofluorescence Antibodies [IgG], Serum) is recommended.

Day(s) and Time(s) Performed

Once or twice weekly, Monday through Friday; 7:30 a.m.- 5 p.m., days of testing to be determined by the laboratory.

Analytic Time

1 day

Specimen Retention Time

14 days

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

83516 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DSG13 Desmoglein 1 and 3, S 43309-4

 

Result ID Test Result Name Result LOINC Value
83680 DSG 1 43311-0
24027 DSG 3 43312-8