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Test Code CD40 B-Cell CD40 Expression by Flow Cytometry, Blood

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Evaluating patients for hyper-IgM type 3 (HIGM3) syndrome due to defects in CD40, typically seen in patients <10 years of age

 

Assessing B-cell immune competence in other clinical contexts, including autoimmunity, malignancy and transplantation

Method Name

Flow Cytometry

Reporting Name

CD40 by Flow, QL, B

Specimen Type

Whole Blood EDTA


Shipping Instructions


Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. Draw and package specimen as close to shipping time as possible.

 

It is recommended that specimens arrive within 24 hours of draw.

 

Specimens arriving on the weekend may be canceled.



Necessary Information


Ordering physician name and phone number are required.



Specimen Required


For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send specimen in original tube. Do not aliquot.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Whole Blood EDTA Ambient 72 hours

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

NA

Other

NA

Reference Values

Present (normal)

Interpretation

This assay is qualitative; CD40 expression is reported as present (normal) or absent (abnormal). Normal B cells express surface CD40 on the majority of cells.

 

Hyper-IgM (HIGM3) syndrome patients typically do not express CD40 on the surface of B cells. Genotyping of CD40 is required for a definite diagnosis of HIGM3. Call 800-533-1710 for ordering assistance.

Day(s) and Time(s) Performed

Monday through Friday

Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.

Analytic Time

3 days

Specimen Retention Time

4 days

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

88184

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CD40 CD40 by Flow, QL, B In Process

 

Result ID Test Result Name Result LOINC Value
89009 CD40 by Flow, QL, B In Process