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Test Code APCRR Activated Protein C Resistance V (APCRV), with Reflex to Factor V Leiden, Plasma

Useful For

Evaluation of patients with incident or recurrent venous thromboembolism (VTE)

 

Evaluation of individuals with a family history of VTE

Profile Information

Test ID Reporting Name Available Separately Always Performed
APCRV Activated Protein Resistance V, P Yes Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
F5DNA Factor V Leiden (R506Q) Mutation, B Yes No
F5DNI APCRV/F5DNA Summary Interpretation No No

Testing Algorithm

If the assay ratio is abnormal, then R506Q mutation will be performed at an additional charge.

 

When the activated protein C resistance V is abnormal or indeterminate and R506Q mutation assay is reflexed, then a summary interpretation will be provided.

Method Name

Optical Clot-Based

Reporting Name

APCRV, w/Reflex, P

Specimen Type

Plasma Na Cit
Whole blood


Specimen Required


See Coagulation Studies in Special Instructions: Guidelines for Specimen Handling and Processing.

 

Both blood and plasma are required.

 

Specimen Type: Whole blood

Container/Tube:

Preferred: Yellow top (ACD)

Acceptable: EDTA or sodium citrate

Specimen Volume: Full tube

Collection Instructions:

1. Invert several times to mix blood.

2. Send specimen in original tube.

Additional Information: Each molecular coagulation test requested must have its own tube.

 

Specimen Type: Platelet-poor plasma

Patient Preparation: Fasting preferred

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Polypropylene vial

Specimen Volume: 1 mL

Collection Instructions:

1. Spin down, remove plasma, and spin plasma again.

2. Freeze plasma aliquots immediately (no longer than 4 hours after collection) at -20° C, or, ideally ≤-40 degrees C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

3. Each coagulation assay requested should have its own vial.


Specimen Minimum Volume

Plasma: 0.5 mL
Whole Blood: 3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days
Whole blood Ambient (preferred) 7 days
  Frozen  14 days
  Refrigerated  14 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

Reference Values

APCRV RATIO

≥2.3

Pediatric reference range has neither been established nor is available in scientific literature. The adult reference range likely would be applicable to children older than 6 months.

Interpretation

An activated protein C (APC) resistance ratio below 2.3 suggests abnormal resistance to APC of hereditary origin.

 

If the screening APC resistance test is abnormal, DNA-based testing for the factor V Leiden mutation factor V Leiden (R506Q) mutation is performed to confirm or exclude hereditary APC-resistance.

Day(s) and Time(s) Performed

Monday through Friday

Analytic Time

4-7 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

85307

LOINC Code Information

Test ID Test Order Name Order LOINC Value
APCRR APCRV, w/Reflex, P 13590-5

 

Result ID Test Result Name Result LOINC Value
APCR APCRV Ratio 13590-5
INT55 Interpretation 48591-2

Forms

1. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) is available in Special Instructions.

2. Coagulation Patient Information (T675) in Special Instructions

3. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.

Test Classification

See Individual Test IDs