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Test Code 0291229 Acute Hepatitis Profile

Additional Codes

 

Mayo Test ID
AHEP

 


Shipping Instructions


 



Necessary Information


Date of draw is required.



Specimen Required


Both 0.5 mL of refrigerated serum and 2.5 mL of frozen serum are preferred for this test.

 

Patient Preparation: For 24 hours before this test, do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Collection Container/Tube: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 3 mL

Collection Instructions:

1. Centrifuge blood collection tube per collection tube manufacturer's instructions.

2. Transfer 0.5 mL serum into an aliquot tube labeled as HAIGM, and ship refrigerate (required).

3. Transfer remaining 2.5 mL serum into a second aliquot tube labeled as SST Serum, and ship frozen (preferred).


Useful For

Differential diagnosis of recent acute viral hepatitis

Profile Information

Test ID Reporting Name Available Separately Always Performed
HAIGM Hepatitis A IgM Ab, S Yes Yes
HBAG HBs Antigen, S Yes Yes
HBIM HBc IgM Ab, S Yes Yes
HCVDX HCV Ab w/Reflex to HCV PCR, S Yes Yes

Testing Algorithm

If hepatitis C virus (HCV) antibody is reactive, then HCV RNA detection and quantification by real-time reverse transcription-PCR will be performed at an additional charge.

 

If hepatitis Bs antigen is reactive, then confirmation will be performed at an additional charge.

 

The following algorithms are available in Special Instructions:

-HBV Infection-Diagnostic Approach and Management Algorithm

-Hepatitis C: Testing Algorithm for Screening and Diagnosis

-Viral Hepatitis Serologic Profiles

Method Name

HAIGM: Chemiluminescent Microparticle Immunoassay (CMIA)

HBAG, HBIM, HCVDX, HBGNT: Chemiluminescence Immunoassay (CIA)

Reporting Name

Acute Hepatitis Profile

Specimen Type

Serum
Serum SST

Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated 5 days
Serum SST Frozen (preferred) 30 days
  Refrigerated  5 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

Plasma, red-top serum, heat-inactivated specimen

Reference Values

HEPATITIS B SURFACE ANTIGEN

Negative

 

HEPATITIS B SURFACE ANTIGEN CONFIRMATION

Negative

 

HEPATITIS B CORE IgM ANTIBODY

Negative

 

HEPATITIS A IgM ANTIBODY

Negative

 

HEPATITIS C ANTIBODY

Negative

 

HEPATITIS C VIRUS RNA DETECTION and QUANTIFICATION by REAL-TIME RT-PCR

Undetected

 

Interpretation depends on clinical setting. See Viral Hepatitis Serologic Profiles in Special Instructions.

Interpretation

Hepatitis A:

Antibody against hepatitis A antigen is usually detectable by the onset of symptoms (usually 15-45 days after exposure). The initial antibody consists almost entirely of IgM subclass antibody. Antibody to hepatitis A virus (anti-HAV) IgM usually falls to undetectable levels 3 to 6 months after infection.

 

Hepatitis B:

Hepatitis B surface antigen (HBsAg) is the first serologic marker appearing in the serum 6 to 16 weeks following hepatitis B virus (HBV) infection. In acute cases, HBsAg usually disappears 1 to 2 months after the onset of symptoms. Hepatitis B surface antibody (anti-HBs) appears with the resolution of HBV infection after the disappearance of HBsAg. Anti-HBs also appears as the immune response following a course of inoculation with the hepatitis B vaccine.

 

Initially, hepatitis B core antibody (anti-HBc) consists almost entirely of the IgM subclass. Anti-HBc, IgM can be detected shortly after the onset of symptoms and is usually present for 6 months. Anti-HBc may be the only marker of a recent HBV infection detectable following the disappearance of HBsAg, and prior to the appearance of anti-HBs, ie, window period.

 

Hepatitis C:

Hepatitis C antibody is usually not detectable during the early months following infection and is almost always detectable by the late convalescent stage of infection. Hepatitis C antibody is not neutralizing and does not provide immunity.

 

If HBsAg, anti-HAV (IgM), and anti-HCV are negative and patient's condition warrants, consider testing for Epstein-Barr virus or cytomegalovirus.

 

The following algorithms are available in Special Instructions:

-HBV Infection-Diagnostic Approach and Management Algorithm

-Hepatitis C: Testing Algorithm for Screening and Diagnosis

Day(s) and Time(s) Performed

Monday through Saturday; Varies

Analytic Time

1 day

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

80074 (if all 4 initial tests are performed)

86709 (if all 4 are not performed)

86705 (if all 4 are not performed)

87340 (if all 4 are not performed)

86803 (if all 4 are not performed)

87522 (if appropriate)

87341 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AHEP Acute Hepatitis Profile 24363-4

 

Result ID Test Result Name Result LOINC Value
HCVA4 HCV Ab, S 13955-0
HBIM HBc IgM Ab, S 24113-3
H_BAG HBs Antigen, S 5196-1
HAIGM Hepatitis A IgM Ab, S 13950-1

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HBGNT HBs Antigen Confirmation, S No No
HCVQN HCV RNA Detect/Quant, S Yes No

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.