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Test Code 0220819 Hematologic Disorders, Fluorescence In Situ Hybridization (FISH) Hold, Bone Marrow or Peripheral Blood

Additional Codes


Mayo Test ID


Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Processing the bone marrow or peripheral blood specimen but delaying FISH analysis while preliminary morphologic assessment is in process

Testing Algorithm

This test is designed to hold the sample and delay FISH testing while preliminary morphologic assessment or flow cytometry testing is in process.


HOLD policy: Upon sample receipt, the specimen will be held in the laboratory. FISH testing will not be performed unless the client contacts the laboratory and indicates that FISH testing is desired. The client must contact the Cytogenetics Laboratory at 507-266-0790 by 4 p.m. (central time) no later than 4 business days (96 hours) after the specimen was collected. If no notification is received by this time, the HOLDF order will be processed as "cancelled". Weekend communication can be deferred until Monday.

Method Name

Direct Preparation of Specimen

Reporting Name

Heme FISH Hold, B/BM

Specimen Type


Specimen Required

Provide a reason for referral with each specimen and bone marrow pathology report (if available). The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.


Advise Express Mail or equivalent if not on courier service.


Submit only 1 of the following specimens:


Specimen Type: Whole blood

Container/Tube: Green top (sodium heparin)

Specimen Volume: 7-10 mL

Collection Instructions:

1. Invert several times to mix blood.

2. Other anticoagulants are not recommended and are harmful to the viability of the cells.


Specimen Type: Bone marrow

Container/Tube: Green top (sodium heparin)

Specimen Volume: 2-3 mL

Collection Instructions:

1. It is preferable to send the first aspirate from the bone marrow collection.

2. Invert several times to mix bone marrow.

3. Other anticoagulants are not recommended and are harmful to the viability of the cells.

Specimen Minimum Volume

Blood: 2 mL
Bone Marrow: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)

Reject Due To

No specimen should be rejected.

Reference Values

Not applicable


If notified by the client, this test may be canceled and a processing fee will be assessed.


If no notification to proceed with testing is received, this test will be reported as "canceled."

Day(s) and Time(s) Performed

Samples processed Monday through Sunday. Results reported Monday through Friday, 8 a.m.-5 p.m.

Analytic Time

4 days

Specimen Retention Time

Original specimen - 4 weeks Remaining fixed cell pellet - Indefinitely

CPT Code Information

See individual reflex tests

LOINC Code Information

Result ID Test Result Name Result LOINC Value
51836 Result Summary 50397-9
51838 Interpretation 69965-2
CG666 Reason for Referral 42349-1
CG667 Specimen 31208-2
51839 Source 31208-2
CG791 Requested FISH Test 48767-8
51841 Method 49549-9
53433 Additional Information 48767-8
51842 Released by 18771-6


If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
MISCF Miscellaneous Studies, FISH Yes No
IMRGF Imatinib Mesylate Resp Genes, FISH Yes No
TALLF ALL (T-cell), FISH Yes No
TLPF T-cell Lymphoma, FISH, B/BM Yes No
FGFRF FGFR1 (8p11.2), FISH Yes No
MNBF MYCN (2p24), FISH, B/BM Yes No
MFCF Myeloma, FISH, Fixed Cells Yes No
BALLF ALL (B-cell), FISH Yes No
BLPF B-cell Lymphoma, FISH, B/BM Yes No
BMTF XX/XY in Opposite Sex BMT, FISH Yes No
CHICF CHIC2 (4q12) Deletion, FISH Yes No
922F BCR/ABL1, FISH Yes No
PCPDF Plasma Cell Prolif, cIg, FISH Yes No