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HelpTest Code 0210674 Activated Protein C Resistance V (APCRV), Plasma
Additional Codes
| Mayo Test ID |
|---|
| APCRV |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Useful For
Evaluation of patients with incident or recurrent venous thromboembolism (VTE)
Evaluation of individuals with a family history of VTE
Special Instructions
Method Name
Optical Clot-Based
Reporting Name
Activated Protein Resistance V, PSpecimen Type
Plasma Na CitAdvisory Information
Although this assay can be performed in the absence of other coagulation tests and clinical information, it is most reliably performed as part of a consultative coagulation test panel with interpretive reporting (including appropriate testing of the same specimen to evaluate for the presence or absence of coagulation abnormalities or conditions that may affect interpretation of the APC resistance assay). This test is included among a panel of tests designated THRMP / Thrombophilia Profile.
Necessary Information
If priority specimen, mark request form, give reason, and request a call-back.
Specimen Required
See Coagulation Studies in Special Instructions: Guidelines for Specimen Handling and Processing.
Specimen Type: Platelet-poor plasma
Collection Container/Tube: Light-blue top (citrate)
Submission Container/Tube: Polypropylene vial
Specimen Volume: 1 mL
Collection Instructions:
1. Spin down, remove plasma
2. Spin plasma again; remove plasma aliquot without disturbing bottom 0.5 mL
3. Freeze specimen aliquot immediately at or below -40° C, if possible but no longer than 4 hours after collection
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. If priority specimen, mark request form, give reason, and request a call-back.
3. Each coagulation assay requested should have its own vial.
Freeze plasma within 2 hours of collection and submit frozen.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Reject Due To
|
Hemolysis |
Mild OK; Gross reject |
|
Lipemia |
Mild OK; Gross reject |
|
Icterus |
Mild OK; Gross reject |
|
Other |
NA |
Reference Values
APCRV RATIO
≥2.3
Pediatric reference range has neither been established nor is available in scientific literature. The adult reference range likely would be applicable to children older than 6 months.
Interpretation
An activated protein C (APC) resistance ratio of less than 2.3 suggests abnormal resistance to APC of hereditary origin.
If the APC resistance test is abnormal, DNA-based testing for the factor V Leiden mutation (F5DNA / Factor V Leiden [R506Q] Mutation, Blood) may be helpful in confirming or excluding hereditary APC-resistance.
Day(s) and Time(s) Performed
Monday through Friday
Analytic Time
1 daySpecimen Retention Time
7 daysTest Classification
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
85307
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| APCRV | Activated Protein Resistance V, P | 13590-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| APCR | APCRV Ratio | 13590-5 |
| INT55 | Interpretation | 48591-2 |
Forms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.